Beyond such fundamental doubts, there remains the tactical question of sequencing.

除了这些基本的质疑以外，还有些在次序上的策略问题。

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However, how to efficiently use and to analyze these massive 454 sequencing data still remains a question.

然而，如何更好地利用454测序并进行后续的数据分析一直是广受关注的问题。

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